Challenges with bioreactor sterile conditions

I’ve been facing some difficulties maintaining sterile conditions in our bioreactor during the seed train phase. It’s crucial, especially when scaling up for the production run. I’m using a new media supplement this time which seems to complicate things. Has anyone else experienced issues with sterility in similar setups?

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, I can totally relate to the frustrations of maintaining sterility, especially when experimenting with new media supplements. We’ve had better luck when we switched to a smaller seed volume to minimize contamination risks during the seed train phase. Have you tried adjusting your inoculation strategy?

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